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dapi staining solution  (Beyotime)


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    Structured Review

    Beyotime dapi staining solution
    Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2029 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution/DAPI+Staining+Solution/pmc13097096-64-0-21
    Average 99 stars, based on 2029 article reviews
    dapi staining solution - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Acod1/itaconate axis controls anxiety-like behaviors induced by chronic infection of Toxoplasma gondii in mice.
    Article Snippet: The secondary antibody was shown as follows: goat-anti-rabbit Cy3 conjugated antibody (gb21303, 1:400, Servicebio). .. After washing with PBS, the second primary antibody was added to the dealt sections for incubation at room temperature for 2 h. Following the incubation, DAPI solution (P0131, Beyotime) was used to counterstain sections. .. Finally, the images were photographed by OLYMPUS IX51 microscope (Tokyo, Japan) and analyzed by the ImageJ software (https://imagej.nih.gov/ij/).

    Article Title: Association between serum neutrophil extracellular traps and carotid intima-media thickness in type 2 diabetes: a cross-sectional study
    Article Snippet: .. Corresponding secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG (GB25303, Servicebio, China) was added and incubated for 1 h at room temperature in the dark.After three PBS washes, cells were stained with DAPI solution (C1002, Beyotime, China) for 10 min at room temperature in the dark to label nuclei.After a final three washes with PBS, cells were mounted with anti-fluorescence quenching mounting medium and imaged under an inverted fluorescence microscope. (b) NE staining only. ..

    Article Title: Acod1/itaconate axis controls anxiety-like behaviors induced by chronic infection of Toxoplasma gondii in mice
    Article Snippet: The secondary antibody was shown as follows: goat-anti-rabbit Cy3 conjugated antibody (gb21303, 1:400, Servicebio). .. After washing with PBS, the second primary antibody was added to the dealt sections for incubation at room temperature for 2 h. Following the incubation, DAPI solution (P0131, Beyotime) was used to counterstain sections. .. Finally, the images were photographed by OLYMPUS IX51 microscope (Tokyo, Japan) and analyzed by the ImageJ software ( https://imagej.nih.gov/ij/ ).

    Staining:

    Article Title: Maresin 1 activates autophagy through SIRT1/PPAR-γ signaling to mitigate high glucose-induced pyroptosis in human retinal pigment epithelial cells
    Article Snippet: The next day, the cells were further incubated with a FITC conjugated goat anti-rabbit IgG secondary antibody (1:1,000; cat. no. GB22303; Wuhan Servicebio Technology Co., Ltd.) at room temperature for 2 h in the dark. .. Finally, the cells were stained with a DAPI solution (1 μg/ml; cat. no. C1002; Beyotime Biotechnology) for 10 min at room temperature in the dark before images were captured using a CKX53 fluorescence microscope. .. Protein extracts were prepared from ARPE-19 cells using RIPA buffer (cat. no. P0013B; Beyotime Biotechnology), and protein concentrations were further quantified using the same BCA protein assay kit as described in the ELISA section.

    Article Title: Small-molecule PCSK9 inhibition enhances BBB amyloid-β clearance and suppresses microglial inflammation in Alzheimer's disease models.
    Article Snippet: Aβ1–42 and 5-TAMRA-labeled Aβ1–42 (5-TAMRA-Aβ1–42) were purchased from Qiangyao Biotechnology (Shanghai, China). .. DMSO, 4% paraformaldehyde (PFA), LysoTracker Green, DAPI solution, proteinase K, acridine orange staining kit, one-step TUNEL apoptosis assay kit (green fluorescence), Hydrogen Peroxide Assay Kit, a BCA protein assay kit and Cell Counting Kit-8 were purchased from Beyotime Biotechnology (Shanghai, China). .. 0.5% Triton X-100, 20 × Tris-EDTA buffer (pH 8.0) and Bovine serum albumin (BSA) were purchased from Servicebio (Wuhan, China).

    Article Title: Association between serum neutrophil extracellular traps and carotid intima-media thickness in type 2 diabetes: a cross-sectional study
    Article Snippet: .. Corresponding secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG (GB25303, Servicebio, China) was added and incubated for 1 h at room temperature in the dark.After three PBS washes, cells were stained with DAPI solution (C1002, Beyotime, China) for 10 min at room temperature in the dark to label nuclei.After a final three washes with PBS, cells were mounted with anti-fluorescence quenching mounting medium and imaged under an inverted fluorescence microscope. (b) NE staining only. ..

    Fluorescence:

    Article Title: Maresin 1 activates autophagy through SIRT1/PPAR-γ signaling to mitigate high glucose-induced pyroptosis in human retinal pigment epithelial cells
    Article Snippet: The next day, the cells were further incubated with a FITC conjugated goat anti-rabbit IgG secondary antibody (1:1,000; cat. no. GB22303; Wuhan Servicebio Technology Co., Ltd.) at room temperature for 2 h in the dark. .. Finally, the cells were stained with a DAPI solution (1 μg/ml; cat. no. C1002; Beyotime Biotechnology) for 10 min at room temperature in the dark before images were captured using a CKX53 fluorescence microscope. .. Protein extracts were prepared from ARPE-19 cells using RIPA buffer (cat. no. P0013B; Beyotime Biotechnology), and protein concentrations were further quantified using the same BCA protein assay kit as described in the ELISA section.

    Article Title: Small-molecule PCSK9 inhibition enhances BBB amyloid-β clearance and suppresses microglial inflammation in Alzheimer's disease models.
    Article Snippet: Aβ1–42 and 5-TAMRA-labeled Aβ1–42 (5-TAMRA-Aβ1–42) were purchased from Qiangyao Biotechnology (Shanghai, China). .. DMSO, 4% paraformaldehyde (PFA), LysoTracker Green, DAPI solution, proteinase K, acridine orange staining kit, one-step TUNEL apoptosis assay kit (green fluorescence), Hydrogen Peroxide Assay Kit, a BCA protein assay kit and Cell Counting Kit-8 were purchased from Beyotime Biotechnology (Shanghai, China). .. 0.5% Triton X-100, 20 × Tris-EDTA buffer (pH 8.0) and Bovine serum albumin (BSA) were purchased from Servicebio (Wuhan, China).

    Article Title: Association between serum neutrophil extracellular traps and carotid intima-media thickness in type 2 diabetes: a cross-sectional study
    Article Snippet: .. Corresponding secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG (GB25303, Servicebio, China) was added and incubated for 1 h at room temperature in the dark.After three PBS washes, cells were stained with DAPI solution (C1002, Beyotime, China) for 10 min at room temperature in the dark to label nuclei.After a final three washes with PBS, cells were mounted with anti-fluorescence quenching mounting medium and imaged under an inverted fluorescence microscope. (b) NE staining only. ..

    Microscopy:

    Article Title: Maresin 1 activates autophagy through SIRT1/PPAR-γ signaling to mitigate high glucose-induced pyroptosis in human retinal pigment epithelial cells
    Article Snippet: The next day, the cells were further incubated with a FITC conjugated goat anti-rabbit IgG secondary antibody (1:1,000; cat. no. GB22303; Wuhan Servicebio Technology Co., Ltd.) at room temperature for 2 h in the dark. .. Finally, the cells were stained with a DAPI solution (1 μg/ml; cat. no. C1002; Beyotime Biotechnology) for 10 min at room temperature in the dark before images were captured using a CKX53 fluorescence microscope. .. Protein extracts were prepared from ARPE-19 cells using RIPA buffer (cat. no. P0013B; Beyotime Biotechnology), and protein concentrations were further quantified using the same BCA protein assay kit as described in the ELISA section.

    Article Title: Association between serum neutrophil extracellular traps and carotid intima-media thickness in type 2 diabetes: a cross-sectional study
    Article Snippet: .. Corresponding secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit IgG (GB25303, Servicebio, China) was added and incubated for 1 h at room temperature in the dark.After three PBS washes, cells were stained with DAPI solution (C1002, Beyotime, China) for 10 min at room temperature in the dark to label nuclei.After a final three washes with PBS, cells were mounted with anti-fluorescence quenching mounting medium and imaged under an inverted fluorescence microscope. (b) NE staining only. ..

    TUNEL Assay:

    Article Title: Small-molecule PCSK9 inhibition enhances BBB amyloid-β clearance and suppresses microglial inflammation in Alzheimer's disease models.
    Article Snippet: Aβ1–42 and 5-TAMRA-labeled Aβ1–42 (5-TAMRA-Aβ1–42) were purchased from Qiangyao Biotechnology (Shanghai, China). .. DMSO, 4% paraformaldehyde (PFA), LysoTracker Green, DAPI solution, proteinase K, acridine orange staining kit, one-step TUNEL apoptosis assay kit (green fluorescence), Hydrogen Peroxide Assay Kit, a BCA protein assay kit and Cell Counting Kit-8 were purchased from Beyotime Biotechnology (Shanghai, China). .. 0.5% Triton X-100, 20 × Tris-EDTA buffer (pH 8.0) and Bovine serum albumin (BSA) were purchased from Servicebio (Wuhan, China).

    Apoptosis Assay:

    Article Title: Small-molecule PCSK9 inhibition enhances BBB amyloid-β clearance and suppresses microglial inflammation in Alzheimer's disease models.
    Article Snippet: Aβ1–42 and 5-TAMRA-labeled Aβ1–42 (5-TAMRA-Aβ1–42) were purchased from Qiangyao Biotechnology (Shanghai, China). .. DMSO, 4% paraformaldehyde (PFA), LysoTracker Green, DAPI solution, proteinase K, acridine orange staining kit, one-step TUNEL apoptosis assay kit (green fluorescence), Hydrogen Peroxide Assay Kit, a BCA protein assay kit and Cell Counting Kit-8 were purchased from Beyotime Biotechnology (Shanghai, China). .. 0.5% Triton X-100, 20 × Tris-EDTA buffer (pH 8.0) and Bovine serum albumin (BSA) were purchased from Servicebio (Wuhan, China).

    H2O2 Assay:

    Article Title: Small-molecule PCSK9 inhibition enhances BBB amyloid-β clearance and suppresses microglial inflammation in Alzheimer's disease models.
    Article Snippet: Aβ1–42 and 5-TAMRA-labeled Aβ1–42 (5-TAMRA-Aβ1–42) were purchased from Qiangyao Biotechnology (Shanghai, China). .. DMSO, 4% paraformaldehyde (PFA), LysoTracker Green, DAPI solution, proteinase K, acridine orange staining kit, one-step TUNEL apoptosis assay kit (green fluorescence), Hydrogen Peroxide Assay Kit, a BCA protein assay kit and Cell Counting Kit-8 were purchased from Beyotime Biotechnology (Shanghai, China). .. 0.5% Triton X-100, 20 × Tris-EDTA buffer (pH 8.0) and Bovine serum albumin (BSA) were purchased from Servicebio (Wuhan, China).

    Bicinchoninic Acid Protein Assay:

    Article Title: Small-molecule PCSK9 inhibition enhances BBB amyloid-β clearance and suppresses microglial inflammation in Alzheimer's disease models.
    Article Snippet: Aβ1–42 and 5-TAMRA-labeled Aβ1–42 (5-TAMRA-Aβ1–42) were purchased from Qiangyao Biotechnology (Shanghai, China). .. DMSO, 4% paraformaldehyde (PFA), LysoTracker Green, DAPI solution, proteinase K, acridine orange staining kit, one-step TUNEL apoptosis assay kit (green fluorescence), Hydrogen Peroxide Assay Kit, a BCA protein assay kit and Cell Counting Kit-8 were purchased from Beyotime Biotechnology (Shanghai, China). .. 0.5% Triton X-100, 20 × Tris-EDTA buffer (pH 8.0) and Bovine serum albumin (BSA) were purchased from Servicebio (Wuhan, China).

    CCK-8 Assay:

    Article Title: Small-molecule PCSK9 inhibition enhances BBB amyloid-β clearance and suppresses microglial inflammation in Alzheimer's disease models.
    Article Snippet: Aβ1–42 and 5-TAMRA-labeled Aβ1–42 (5-TAMRA-Aβ1–42) were purchased from Qiangyao Biotechnology (Shanghai, China). .. DMSO, 4% paraformaldehyde (PFA), LysoTracker Green, DAPI solution, proteinase K, acridine orange staining kit, one-step TUNEL apoptosis assay kit (green fluorescence), Hydrogen Peroxide Assay Kit, a BCA protein assay kit and Cell Counting Kit-8 were purchased from Beyotime Biotechnology (Shanghai, China). .. 0.5% Triton X-100, 20 × Tris-EDTA buffer (pH 8.0) and Bovine serum albumin (BSA) were purchased from Servicebio (Wuhan, China).

    Article Title: FAP-targeting peptide-directed nanoprobes enable tumor microenvironment-activatable MR/NIRF imaging of breast cancer primary tumor and lung metastases
    Article Snippet: Gd-DOTA was purchased from Adamas-beta Co., Ltd (Shanghai, China). .. The BCA protein concentration assay kit, enhanced CCK8 assay kit, D-Luciferin potassium salt, Lyso-Tracker, Mito-Tracker, anti-GAPDH antibody, HRP goat anti-rabbit IgG(H + L), Cy3-conjugated goat anti-rabbit IgG(H + L), and DAPI solution were all obtained from Beyotime (Shanghai, China). ..

    Protein Concentration:

    Article Title: FAP-targeting peptide-directed nanoprobes enable tumor microenvironment-activatable MR/NIRF imaging of breast cancer primary tumor and lung metastases
    Article Snippet: Gd-DOTA was purchased from Adamas-beta Co., Ltd (Shanghai, China). .. The BCA protein concentration assay kit, enhanced CCK8 assay kit, D-Luciferin potassium salt, Lyso-Tracker, Mito-Tracker, anti-GAPDH antibody, HRP goat anti-rabbit IgG(H + L), Cy3-conjugated goat anti-rabbit IgG(H + L), and DAPI solution were all obtained from Beyotime (Shanghai, China). ..



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